I work in a hospital lab. My educational background is just a BS in biochemistry, so I'm familiar with the techniques you posted. I've been working in a clinical lab for several years now.

I'll be a bit blunt here. What we use are analyte specific chemiluminescent assays to determine concentrations of analytes in serum. We don't use NMR, although one of our sub-labs that monitors metabolic deficiencies (usually in newborns) does use a HPLC-tandem mass spectrometer.

The easiest way to think about the real world version of this is working with a hundred-thousand dollar Easy-Bake Oven. A manufacturer will supply you with what you need, such as QC, reagent, the instrument and you load everything on correctly. You place the serum/plasma/urine/whatever onto the instrument and it will eventually give you a read out so long as the QC passes which validates the result.